polyclonal rabbit anti syndecan 1  (Danaher Inc)

Journal: Arthritis Research & Therapy

Article Title: Characterization of inflammatory cell infiltrate of scleroderma skin: B cells and skin score progression

doi: 10.1186/s13075-018-1569-0

Figure Lengend Snippet: CD68 + , CD3 + , CD20 + and CD138 + cell counts on paired skin specimens in the 28 patients with SSc

Article Snippet: Sections were incubated with anti-CD3 mouse monoclonal antibodies (mAb) (1:50 dilution; Clone PS1, Abcam, Cambridge, MA, USA) or anti-CD20 mouse mAb (1:100 dilution; Clone L26; Novus Biologicals, Littleton, CO, USA) or human anti-CD138 (Syndecan-1) rabbit polyclonal antibodies (Ab) (1:25 dilution; Spring Bioscience, CA, USA).

Techniques:

Journal: Arthritis Research & Therapy

Article Title: Characterization of inflammatory cell infiltrate of scleroderma skin: B cells and skin score progression

doi: 10.1186/s13075-018-1569-0

Figure Lengend Snippet: CD138 cell staining in involved and uninvolved skin from a patient with systemic sclerosis (SSc). a Involved skin (distal forearm biopsy) from a patient with an early anti-Scl-70 + diffuse SSc showing an appreciable number of CD138 + cells in perivascular areas. b Uninvolved skin (buttock biopsy) of the same patient showing the presence of a smaller number of CD138 + cells around blood vessels. Original magnification × 400 ( a and b ). Nuclei were counterstained by hematoxylin. Bottom insets show the infiltrating cells at a lower magnification (× 200). Insets at the top of the figures show hematoxylin and eosin (H&E) staining of involved and uninvolved skin (× 50)

Article Snippet: Sections were incubated with anti-CD3 mouse monoclonal antibodies (mAb) (1:50 dilution; Clone PS1, Abcam, Cambridge, MA, USA) or anti-CD20 mouse mAb (1:100 dilution; Clone L26; Novus Biologicals, Littleton, CO, USA) or human anti-CD138 (Syndecan-1) rabbit polyclonal antibodies (Ab) (1:25 dilution; Spring Bioscience, CA, USA).

Techniques: Staining

Journal: Arthritis Research & Therapy

Article Title: Characterization of inflammatory cell infiltrate of scleroderma skin: B cells and skin score progression

doi: 10.1186/s13075-018-1569-0

Figure Lengend Snippet: Clinical and histological characteristics according to immunosuppressive/immunomodulatory treatment and clinical outcome in the 28 patients with SSc

Article Snippet: Sections were incubated with anti-CD3 mouse monoclonal antibodies (mAb) (1:50 dilution; Clone PS1, Abcam, Cambridge, MA, USA) or anti-CD20 mouse mAb (1:100 dilution; Clone L26; Novus Biologicals, Littleton, CO, USA) or human anti-CD138 (Syndecan-1) rabbit polyclonal antibodies (Ab) (1:25 dilution; Spring Bioscience, CA, USA).

Techniques: